| Pole | KOD | Znaczenie kodu |
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| Zamierzone efekty uczenia się | BTap_2A_BTA-S2-D11_K01 | Student is aware that there is a number of methods to analyse the different levels of protein changes in response to various physiological/patophysiological stimmuli in the biological material. |
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| Odniesienie do efektów kształcenia dla kierunku studiów | BTap_2A_K01 | demonstrates the need to constantly improve general and directional knowledge; is aware of the purposefulness of raising the acquired knowledge both in professional activities and personal development |
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| BTap_2A_K02 | demonstrates understanding of biotechnological processes used in various areas of human activity; interprets and describes these processes using a scientific approach |
| BTap_2A_K05 | shows discipline in individual work; willing to participate in group work; can creatively plan and implement own and team activities |
| Cel przedmiotu | C-1 | Theoretical and practical knowledge of gel-based and chromatographic protein separation techniques. |
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| C-2 | The ability of the participants to use advanced bioinformatic tools to analyse proteomic data (1-D and 2-D gels, mass spectra). |
| C-3 | Practical use of MALDI-TOF MS (matrix-assisted laser desorption/ionisation time of flight mass spectrometer) for protein identification. |
| Treści programowe | T-L-6 | 1-DE and 2-DE gel image acquisition and bioinformatic analysis. |
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| T-L-1 | Sample preparation techniques for proteomic analysis. |
| T-L-4 | Protein separation using two-dimensional electrophoresis (2-DE). |
| T-L-2 | Protein separation using SDS-PAGE (1-DE). |
| T-L-5 | Protein gel staining methods. |
| T-L-7 | Identification of proteins using mass spectrometer MALTI-TOF. |
| T-L-3 | Identification of proteins using Western-blot technique. |
| T-W-3 | Gel-based protein separation techniques. The components of resolving gel matrix. Sodium-dodecyl polyacrylamide gel electrophoresis (SDS-PAGE), the principle and application of native PAGE electrophoresis. Two dimensional electrophoresis (2-DE) – the principle of the method, sample preparation for 2-DE, IPG strips, isoelectric focusing. |
| T-W-4 | Protein detection methods: coomassie stain, silver stain, negative ion staining (copper, zinc), autoradiography, fluorography, fluorescent staining. Two-dimensional difference in gel electrophoresis (2D-DIGE) – the principle and application of the method. Image acquisition and analysis of 1-D and 2-D gels. 1-D and 2-D gels analysis softwares. |
| T-W-5 | Application of mass spectrometry (MS) for protein identification. Ionization methods in mass spectrometry. Types of mass analyzers. Peptide mass fingerprinting (PMF). |
| T-W-6 | Identification of proteins using Western-blot technique. Sample preparation. Methods of protein transfer. Incubation with antibodies. Visualisation. |
| T-W-1 | Introduction to proteomics. Biological significance of post-transcriptional and post-translational protein modifications. Proteome organization. The general principles of proteomic analysis. |
| T-W-2 | Collection, preparation and storage of biological material for proteomic analyses. Cell lysis, composition of lysis buffers (chaotropic agents, detergents, reducing agents, ampholytes), methods of purification of complex biological samples, methods of protein precipitation. |
| T-W-7 | Main branches of proteomics: structural, quantitative, functional and clinical. Proteomics in the diagnosis and prognosis of biological processes - innovative tools for learning about the body's activities in the state of health and the disease process. The importance of identification and characterization of proteins and differences in protein profiles depending on age, physiological state, drug effects, etc. The use of proteomics to produce new, highly selective drugs that allow for individual therapy. The possibility of using proteomics to solve practical problems in the areas of: biology, biotechnology, agriculture, medicine, health care, forensic science and others. |
| T-W-8 | Identification and localization of post-translational modifications. Post-translational modifications of proteins and their importance. Peptide sequence identification methods. Methods of locating sites of post-translational modifications. |
| T-W-9 | Nutriproteomics as a new direction in nutritional research. What is nutriproteomics - assumptions, strategies, areas of interest. Analytical techniques used in nutriproteomics. Functional foods and nutriproteomics. Molecular mechanisms of action of bioactive dietary components. The influence of dietary components on the regulation of protein expression. Research examples. New directions and further perspectives of research in the field of nutriproteomics. |
| T-L-8 | Bioinformatic analysis of proteomic research results. |
| Metody nauczania | M-1 | Theoretical lectures. |
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| M-2 | Discussion during laboratory classes. |
| M-3 | Project preparation. |
| Sposób oceny | S-1 | Ocena formująca: Project presentation in the writing form. |
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| S-2 | Ocena podsumowująca: Writing test. |
| Kryteria oceny | Ocena | Kryterium oceny |
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| 2,0 | |
| 3,0 | Student shows a moderate interest in participating in a verbal discussion with the teacher and colleagues during the classes. |
| 3,5 | |
| 4,0 | |
| 4,5 | |
| 5,0 | |