Wydział Biotechnologii i Hodowli Zwierząt - Biotechnologia (S2)
Sylabus przedmiotu Enzyme engineering:
Informacje podstawowe
| Kierunek studiów | Biotechnologia | ||
|---|---|---|---|
| Forma studiów | studia stacjonarne | Poziom | drugiego stopnia |
| Tytuł zawodowy absolwenta | magister inżynier | ||
| Obszary studiów | charakterystyki PRK, kompetencje inżynierskie PRK | ||
| Profil | ogólnoakademicki | ||
| Moduł | — | ||
| Przedmiot | Enzyme engineering | ||
| Specjalność | Biotechnology in animal production and environmental protection | ||
| Jednostka prowadząca | Katedra Mikrobiologii i Biotechnologii | ||
| Nauczyciel odpowiedzialny | Radosław Drozd <Radoslaw.Drozd@zut.edu.pl> | ||
| Inni nauczyciele | |||
| ECTS (planowane) | 4,0 | ECTS (formy) | 4,0 |
| Forma zaliczenia | egzamin | Język | angielski |
| Blok obieralny | — | Grupa obieralna | — |
Formy dydaktyczne
Wymagania wstępne
| KOD | Wymaganie wstępne |
|---|---|
| W-1 | The basic knowledge of organic and inorganic chemistry, biochemistry, biophysics, physical chemistry, English at intermediate level |
Cele przedmiotu
| KOD | Cel modułu/przedmiotu |
|---|---|
| C-1 | Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes |
| C-2 | Introduction to methods of in silico prediction of protein structures |
| C-3 | Mastering the skills of the selection of appropriate methods for enzyme immobilization |
Treści programowe z podziałem na formy zajęć
| KOD | Treść programowa | Godziny |
|---|---|---|
| ćwiczenia audytoryjne | ||
| T-A-1 | The industrial application of the enzymes from the oxidoreductase classes | 2 |
| T-A-2 | The industrial application of the enzymes from the transferase classes | 2 |
| T-A-3 | The industrial application of the enzymes from the hydrolase classes | 2 |
| T-A-4 | The industrial application of the enzymes from the lyase classes | 2 |
| T-A-5 | The industrial application of the enzymes from the isomerase classes | 2 |
| T-A-6 | The industrial application of the enzymes from the ligase classe | 2 |
| T-A-7 | Optimization of the properties of enzymes for use in industrial processes | 3 |
| 15 | ||
| laboratoria | ||
| T-L-1 | Methods of visualization of protein structures with using the specialized software for molecular modeling | 2 |
| T-L-2 | The computational methods for protein structure determination on the their second and third level organizationji | 4 |
| T-L-3 | Modeling the catalytic properties of the enzymes | 2 |
| T-L-4 | Methods of the enzymes isolation and purification | 2 |
| T-L-5 | Methods for the immobilization of enzymes on the organic and inorganic matrices | 5 |
| 15 | ||
| wykłady | ||
| T-W-1 | The function and significance of the structure of enzymes. The impact amino acid molecular interaction on the stabilization the structure of enzymatic proteins. Methods for the analysis of the primary structure of the enzyme proteins. Methods for the analysis of secondary and tertiary level structure of the enzyme proteins | 2 |
| T-W-2 | The methods of exploring structural and sequence protein databases. The specialist molecular modeling software; practical approach to the comparison of the structure I, II and III the order of the enzymes. Methods of solving the 3D structure of enzymes. Classical methods (X-ray, NMR) vs. comparative modeling and ab-initio methods | 2 |
| T-W-3 | Catalytic properties of enzymes. Methods of the in-vitro study of the enzymes active site properities | 2 |
| T-W-4 | Modeling and optimization of kinetic parameters of enzymes. Prediction and design the catalytic properties of enzymes by in-silico methods. | 2 |
| T-W-5 | The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources | 2 |
| T-W-6 | Microbial production of enzymes with different properties. Types of fermentation processes. Design and optimization of bioreactors for the production of the enzymes on an industrial scale | 2 |
| T-W-7 | The enzymes in food and pharmaceutical industries. The enzymes in the textile and material | 2 |
| T-W-8 | The application of enzymes in environmental remediation and fuel production from renewable sources. he application of enzyme in the household | 1 |
| 15 | ||
Obciążenie pracą studenta - formy aktywności
| KOD | Forma aktywności | Godziny |
|---|---|---|
| ćwiczenia audytoryjne | ||
| A-A-1 | participation in classes | 15 |
| A-A-2 | preparation for classes | 5 |
| A-A-3 | reading the indicated literature | 10 |
| 30 | ||
| laboratoria | ||
| A-L-1 | participation in classes | 15 |
| A-L-2 | preparation for laboratory classes | 10 |
| A-L-3 | reading the indicated literature | 5 |
| 30 | ||
| wykłady | ||
| A-W-1 | preparation for course passing | 15 |
| A-W-2 | participation in classes | 15 |
| A-W-3 | reading the indicated literature | 5 |
| A-W-4 | preparation for classes | 10 |
| A-W-5 | contact hours | 15 |
| 60 | ||
Metody nauczania / narzędzia dydaktyczne
| KOD | Metoda nauczania / narzędzie dydaktyczne |
|---|---|
| M-1 | information lecture |
| M-2 | narrative lectures |
| M-3 | anecdote |
| M-4 | conversational lecture |
Sposoby oceny
| KOD | Sposób oceny |
|---|---|
| S-1 | Ocena podsumowująca: Written exam, test |
| S-2 | Ocena formująca: Report from laboratory classes Multimedia presentation |
Zamierzone efekty uczenia się - wiedza
| Zamierzone efekty uczenia się | Odniesienie do efektów kształcenia dla kierunku studiów | Odniesienie do efektów zdefiniowanych dla obszaru kształcenia | Odniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżyniera | Cel przedmiotu | Treści programowe | Metody nauczania | Sposób oceny |
|---|---|---|---|---|---|---|---|
| BT_2A_BTZ-A-D8_W01 Student has a knowledge of the scope of structural dependencies shaping the catalytic properties of enzymes. Student knows bioinforamtics tools that allow prediction of the enzyme structure. | BTap_2A_W09 | — | — | C-1, C-2, C-3 | T-L-2, T-L-3 | M-3, M-1, M-4, M-2 | S-1, S-2 |
| BT_2A_BTZ-A-D8_W02 Student knows the methods of enzyme immobilization and methods preparation of highly purified enzyme preparations. | BTap_2A_W09 | — | — | C-1, C-2, C-3 | T-L-4, T-L-5, T-W-1, T-W-4, T-W-5 | M-3, M-1, M-4, M-2 | S-1, S-2 |
| BT_2A_BTZ-A-D8_W03 Student knows the methods of selection of microorganisms for application tasks aimed at the production of species with specific catalytic activity | BTap_2A_W09 | — | — | C-1, C-2, C-3 | T-A-5, T-A-1, T-A-2, T-A-3, T-A-4, T-A-6, T-A-7, T-L-5, T-W-5, T-W-6, T-W-8 | M-3, M-1, M-4, M-2 | S-1, S-2 |
Zamierzone efekty uczenia się - umiejętności
| Zamierzone efekty uczenia się | Odniesienie do efektów kształcenia dla kierunku studiów | Odniesienie do efektów zdefiniowanych dla obszaru kształcenia | Odniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżyniera | Cel przedmiotu | Treści programowe | Metody nauczania | Sposób oceny |
|---|---|---|---|---|---|---|---|
| BT_2A_BTZ-A-D8_U01 Student use bioinformatic tools to solve the enzyme structure, is able to gain dependence between their structure and catalytic functions | BTap_2A_U07 | — | — | C-1, C-2, C-3 | T-L-2, T-L-1, T-L-3, T-W-4, T-W-5 | M-3, M-1, M-4, M-2 | S-2 |
| BT_2A_BTZ-A-D8_U02 Student analyze methods that allow enzyme immoblizations, calculates the possibilities of their application in specific applications | BTap_2A_U07 | — | — | C-1, C-2, C-3 | T-A-7, T-L-5, T-W-4, T-W-5, T-W-6, T-W-7 | M-3, M-1, M-4, M-2 | S-1, S-2 |
Zamierzone efekty uczenia się - inne kompetencje społeczne i personalne
| Zamierzone efekty uczenia się | Odniesienie do efektów kształcenia dla kierunku studiów | Odniesienie do efektów zdefiniowanych dla obszaru kształcenia | Odniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżyniera | Cel przedmiotu | Treści programowe | Metody nauczania | Sposób oceny |
|---|---|---|---|---|---|---|---|
| BT_2A_BTZ-A-D8_K01 Student knows and uses biotechnology methods to improve the quality of life for himself and others | BTap_2A_K02, BTap_2A_K03, BTap_2A_K04 | — | — | C-1, C-2, C-3 | T-A-7, T-W-8 | M-3, M-1, M-4, M-2 | S-1, S-2 |
Kryterium oceny - wiedza
| Efekt uczenia się | Ocena | Kryterium oceny |
|---|---|---|
| BT_2A_BTZ-A-D8_W01 Student has a knowledge of the scope of structural dependencies shaping the catalytic properties of enzymes. Student knows bioinforamtics tools that allow prediction of the enzyme structure. | 2,0 | |
| 3,0 | Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 | ||
| BT_2A_BTZ-A-D8_W02 Student knows the methods of enzyme immobilization and methods preparation of highly purified enzyme preparations. | 2,0 | |
| 3,0 | Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 | ||
| BT_2A_BTZ-A-D8_W03 Student knows the methods of selection of microorganisms for application tasks aimed at the production of species with specific catalytic activity | 2,0 | |
| 3,0 | Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 |
Kryterium oceny - umiejętności
| Efekt uczenia się | Ocena | Kryterium oceny |
|---|---|---|
| BT_2A_BTZ-A-D8_U01 Student use bioinformatic tools to solve the enzyme structure, is able to gain dependence between their structure and catalytic functions | 2,0 | |
| 3,0 | Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 | ||
| BT_2A_BTZ-A-D8_U02 Student analyze methods that allow enzyme immoblizations, calculates the possibilities of their application in specific applications | 2,0 | |
| 3,0 | Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 |
Kryterium oceny - inne kompetencje społeczne i personalne
| Efekt uczenia się | Ocena | Kryterium oceny |
|---|---|---|
| BT_2A_BTZ-A-D8_K01 Student knows and uses biotechnology methods to improve the quality of life for himself and others | 2,0 | |
| 3,0 | Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 |
Literatura podstawowa
- Yoo, Y.J., Feng, Y., Kim, Y.-H., Yagonia, C., Fundamentals of Enzyme Engineering, Springer Netherlands, 2017
- Wolfgang Aehle red., Enzymes in Industry: Production and Applications, Willey VCH, 2007, III
- Allan Svendsen, Enzyme Functionality: Design, Engineering and Screening, 2004
- Christoph Wittmann i Rainer Krull red., Biosystems Engineering I: Creating Superior Biocatalysts, Tom 1, Springer, 2010
- Girish Shukla i Ajit Varma, Soil Enzymology, Springer, 2011
Literatura dodatkowa
- Athel Cornish-Bowden, Fundamentals of Enzyme Kinetics, Portland Press, Londyn, 2002, III